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The concept of transmembrane proteins was obtained from the results of which technique?

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The concept of transmembrane proteins was obtained from the results of which technique?

(a) Freeze-fraction replication

(b) Freeze-fracture replication

(c) Fraction replication

(d) None of the mentioned

This question was posed to me in a national level competition.

My question is from Cellular Membranes topic in portion Cellular Membranes of Cell Biology

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The correct option is (b) Freeze-fracture replication

The explanation: The concept that transmembrane proteins penetrate through the membrane was obtained from the results of freeze-fracture replication. In this procedure, a frozen solid tissue is fractured with a knife blade and metals are deposited on their exposed surfaces to form shadowed replica. This is viewed under electron microscope and it looks like a path strewn with pebbles, called as membrane-associated particles.

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