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In total internal reflection microscopy (TIRF), thin planes can be focused

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In total internal reflection microscopy (TIRF), thin planes can be focused

(a) True

(b) False

The question was asked in unit test.

My question is taken from Studying the Cytoskeleton in chapter Cytoskeleton and Cell Motility of Cell Biology

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Right answer is (a) True

Easiest explanation: Total internal reflection microscopy (TIRF) is a specialized type of laser-based fluorescence microscopy, which allows the focusing of very thin planes. As a result, fluorescent light rays emanating from the field of view do not obscure the specific proteins being monitored.

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