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To make the recombinant plasmid permeable to DNA molecules, which of the chemicals is added?

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To make the recombinant plasmid permeable to DNA molecules, which of the chemicals is added?

(a) MgCl2

(b) CaCl2

(c) NaCl

(d) HCl

I have been asked this question in my homework.

This intriguing question originated from DNA Cloning: The Basics-1 in portion DNA-Based Information Technologies of Biochemistry

1 Answer

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Best answer
Right choice is (b) CaCl2

To elaborate: The cells are treated with CaCl2 to make them permeable to DNA molecules through a process called transformation.

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