Question

GFP joined at the end of a protein can interfere in its synthesis.

(a) True

(b) False

I had been asked this question in an international level competition.

My doubt stems from Cytoplasmic Membrane Systems in division Cytoplasmic Membrane Systems of Cell Biology

Answer 1

The correct answer is (b) False

Easy explanation: GFP is a fluorescent protein used to tag the protein whose biosynthetic pathway has to be determined. A chimeric of GFP synthesizing and test protein synthesizing gene is incorporated in the cell. GFP joined at the end the protein has absolutely no effect on the protein’s activity or pathway.