Question

Which of the following is the correct Line weaver-Burk equation?

(a) \(\frac{1}{V_0} = \frac{K_m}{V_{max} [S]} + \frac{1}{V_{max}} \)

(b) \(\frac{1}{V_{max}} = \frac{K_m}{V_0 [S]} + \frac{1}{V_0} \)

(c) \(V_0 = \frac{V_{max} [S]}{K_m+[S]} \)

(d) \(V_{max} = \frac{V_0⁡ [S]}{K_m+[S]} \)

I had been asked this question in quiz.

I'm obligated to ask this question of Enzyme Kinetics as an Approach to Understanding Mechanism topic in chapter Enzymes, Carbohydrates and Gycobiology of Biochemistry

Answer 1

Right option is (a) \(\frac{1}{V_0} = \frac{K_m}{V_{max} [S]} + \frac{1}{V_{max}} \)

For explanation: Line weaver-Burk equation: \(\frac{1}{V_0} = \frac{K_m}{V_{max} [S]} + \frac{1}{V_{max}} \)

Michaelis-Menten equation: \(V_0 = \frac{V_{max} [S]}{K_m+[S]} \)