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Recombinant DNA construction involves ____________

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Recombinant DNA construction involves ____________

(a) Cleaving DNA with a restriction endonuclease and joining with ligase

(b) Cleaving DNA with ligase and joining with endonuclease

(c) Cleaving and joining DNA with restriction endonuclease

(d) Cleaving DNA with a restriction endonuclease and joining with polymerase

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This question is from From Genes to Genomes topic in portion DNA-Based Information Technologies of Biochemistry

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Right answer is (a) Cleaving DNA with a restriction endonuclease and joining with ligase

Easiest explanation: Restriction enzymes cleave plasmid vector and then DNA ligase joins foreign DNA with cloning vector.

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