Question

Which of the following is true about tm?

(a) It can be termed as renaturation temperature

(b) The higher the content of G ≡ C base pairs, the higher the tm

(c) The higher the content of A = T base pairs, the higher the tm

(d) The higher the content of G ≡ C base pairs, the lower the tm

The question was asked at a job interview.

My question is from Nucleic Acid Chemistry topic in portion Nucleotides and Nucleic Acids of Biochemistry

Answer 1

Right choice is (b) The higher the content of G ≡ C base pairs, the higher the tm

The best I can explain: Tm is the melting temperature or denaturation temperature.