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Which of the following reactions is required for proofreading during DNA replication by DNA polymerase III?

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Which of the following reactions is required for proofreading during DNA replication by DNA polymerase III?

(a) 5’ to 3’ exonuclease activity

(b) 3’ to 5’ exonuclease activity

(c) 3’ to 5’ endonuclease activity

(d) 5’ to 3’ endonuclease activity

The question was asked in semester exam.

Enquiry is from DNA Replication in division DNA, RNA and Protein Metabolism of Biochemistry

1 Answer

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Best answer
Correct choice is (b) 3’ to 5’ exonuclease activity

To explain: The 3’ → 5’ exonuclease activity removes the mispaired nucleotide and the polymerase begins again. This activity is known as proofreading.

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