Question

The presence of these codons at The beginning of the frame _____ give a clear indication of the translation initiation site.

(a) always

(b) does not necessarily

(c) does not

(d) never

I had been asked this question in final exam.

My question is from Gene Prediction in Prokaryotes topic in portion Gene and Promoter Prediction of Bioinformatics

Answer 1

Right option is (b) does not necessarily

To explain I would say: Because there may be multiple ATG, GTG, or TGT codons in a frame, the presence of these codons at the beginning of the frame does not necessarily give a clear indication of the translation initiation site. Instead, to help identify this initiation codon, other features associated with translation are used.