Question

Which of the given statements is incorrect about Clustering by single linkage?

(a) In First step, a group of related sequences found in the all-against-all proteome comparison is subjected to a multiple sequence alignment usually by CLUSTALW

(b) A neighbor-joining algorithm is rarely used in this method

(c) This procedure and the algorithms are the same as those used to make a phylogenetic tree by the distance methods

(d) A distance matrix that shows the number of amino acid changes between each pair of sequences is made

This question was addressed to me at a job interview.

My query is from Comparative Genomics topic in division Collecting & Storing Sequences in Laboratory of Bioinformatics

Answer 1

Correct choice is (b) A neighbor-joining algorithm is rarely used in this method

For explanation I would say: The matrix is then used to cluster the sequences by a neighbor-joining algorithm. These methods produce a tree or a different representation of the tree called a dendrogram, which minimizes the number of amino acid changes that would generate the group of sequences.