Question

In sample holders, for such applications, a 50 cm path-length with about a ________ volume cell is employed.

(a) 0.3 ml

(b) 3 liters

(c) 0.3 liters

(d) 3 ml

The question was asked in examination.

This is a very interesting question from Methods of Monitoring Foetal Heart Rate in portion Foetal Monitoring Instruments of Biomedical Instrumentation

Answer 1

The correct option is (c) 0.3 liters

To explain I would say: Studies of dilute or weakly-absorbing liquid samples, or of samples where trace components must be detected, require a cell with a long path-length. For such applications, a 50 cm path-length with about a 300 ml volume cell is employed.