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Which following is not constant for the calibration factor in Coulter counter?

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Which following is not constant for the calibration factor in Coulter counter?

(a) Electrolyte resistivity

(b) Amplifier gain setting

(c) Given aperture size

(d) Setting of the threshold level control

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My query is from Transmission of Video Images in division Telemedicine Technology of Biomedical Instrumentation

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Right option is (d) Setting of the threshold level control

For explanation: The calibration factor is constant for given aperture size, electrolyte resistivity and amplifier gain setting. It is used for the conversion of threshold settings to particle volumes or their cube roots to equivalent spherical diameters.

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