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Which of the following type is of the perfusion culture?

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Which of the following type is of the perfusion culture?

(a) Batch

(b) Conc. Batch

(c) Continuous

(d) Conc. Fed-Batch

The question was posed to me in examination.

Origin of the question is Ideal Reactor Operation in portion Reactor Engineering of Bioprocess Engineering

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The correct answer is (d) Conc. Fed-Batch

For explanation: Concentrated Fed-Batch- In a process that can be considered a form of perfusion, the culture is perfused to generate ultrahigh cell concentration, greater than 10^8 cells/mL; and the product is also retained in the vessel.

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