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What is Hepes?

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What is Hepes?

(a) An animal culture

(b) A buffer

(c) A type of non-ionic element

(d) A horse serum

This question was addressed to me in an interview for internship.

The above asked question is from Medium Optimization topic in chapter Introduction to Fermentation Processes & Media for Industrial Fermentation of Bioprocess Engineering

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The correct option is (b) A buffer

The explanation is: HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic organic chemical buffering agent; one of the twenty Good’s buffers. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture.

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