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You designed an antibody against phosphorylated CTD of RNA polymerase II. Then you use a DNA fragment with lac operon and try to immunoprecipitate the RNA pol II. What will be the expected result?

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You designed an antibody against phosphorylated CTD of RNA polymerase II. Then you use a DNA fragment with lac operon and try to immunoprecipitate the RNA pol II. What will be the expected result?

(a) Moderate concentration of RNA pol II will precipitate

(b) RNA pol II that precipitated will be contaminated with pol III

(c) High amount of RNA pol II will precipitate

(d) No RNA pol II will precipitate

This question was addressed to me in semester exam.

Enquiry is from Transcription in Eukaryotes : RNA Polymerases in section Gene Expression : Transcription Prokaryotes of Cytogenetics

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The correct answer is (d) No RNA pol II will precipitate

The best explanation: Lac operon is a prokaryotic gene not recognized by eukaryotic RNA polymerase. Thus it will not be transcribing. Non-Transcribing RNA pol II doesn’t have phosphorylated residue in CTD, so it can’t be immunoprecipitated with the given antibody.

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