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In a stretch of DNA being transcribed, the region forward to the Elongation is __________

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In a stretch of DNA being transcribed, the region forward to the Elongation is __________

(a) Positively supercoiled

(b) Negatively supercoiled

(c) Uncoiled

(d) Forms a secondary structure

I have been asked this question in my homework.

This question is from Transcription in Prokaryotes : Elongation topic in section Gene Expression : Transcription Prokaryotes of Cytogenetics

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Correct choice is (a) Positively supercoiled

Best explanation: The region forward to the RNA polymerase elongation machinery is positively supercoiled due to the strain. If the gyrase doesn’t release this strain the polymerizing machinery will stall. Such regions can’t be uncoiled or folded.

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