Question

Which factors lead to disassociation of the ribosomal subunits?

(a) RRF and Ef-G

(b) IF1 and IF2

(c) Rf1 and RRF

(d) IF-1 and IF-3

The question was asked in a national level competition.

I would like to ask this question from Elongation and Termination in division Gene Expression : Translation of Cytogenetics

Answer 1

Correct choice is (d) IF-1 and IF-3

To explain: While the release factor releases the ribosome from the termination complex, in order to keep the ribosome functional the 50S and 30S subunits should be separated. This is maintained by IF1 and IF3 (mainly by IF3) which prevents re-association.