Question

In mice you place are protein under an inducible promoter and you insert the gene under study in the loxP flanking region between opposite segments. If this tissue is induced which of the following will you see?

(a) There will be no visible result

(b) The gene under study will be deleted from specific induced cell

(c) The gene under study will be deleted from all other than the induced cell

(d) The gene will be inserted in the Cre gene sequence

This question was addressed to me in examination.

The above asked question is from Site-Specific Recombination in section Genetic Recombination of Cytogenetics

Answer 1

Right option is (b) The gene under study will be deleted from specific induced cell

Easy explanation: This method is used to selectively delete some genes from some tissues in vivo. In this case the Cre gene is activated when an induction signal is provided and the Cre protein so formed acts as a trans factor on LoxP sequence to remove the flanking gene of interest.