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How many rounds of replication in BrdU will be needed to visualize Harlequin chromosome?

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How many rounds of replication in BrdU will be needed to visualize Harlequin chromosome?

(a) Single

(b) Two

(c) Three

(d) Four

This question was addressed to me in an interview for internship.

My question is taken from Linkage and Crossing Over in portion Genetic Recombination of Cytogenetics

1 Answer

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Correct answer is (b) Two

To explain I would say: Due to the semiconservative nature of DNA replication in the 1^st round the chromosome will have only one parental strand and one strand with BrdU. When two rounds are complete one chromosome had only BrdU strand and other has parental type. The BrdU strand is lighter so distinguishes the appearance.

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