Question

The 5’ end of the tRNA transcript is cleaved by____________

(a) RNase K

(b) Exonuclease VII

(c) Endonucleace I

(d) Rnase P

This question was addressed to me in an online interview.

Query is from RNA Processing Control in section Regulating Gene Expression in Eukaryotes of Cytogenetics

Answer 1

Right option is (d) Rnase P

To explain I would say: RNase P removes the 5’ nucleotide sequence or the tRNA transcript. This generates a 5’ end that is shorter than the 3’ end.