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What is the order of the gene where columns are for different HFR and values denote time?

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What is the order of the gene where columns are for different HFR and values denote time?

(a) Azi, Lac, Gal, Ton

(b) Lac, Gal, Azi, Ton

(c) Gal, Lac, Ton, Azi

(d) Tob, Azi, Lac, Gal

I had been asked this question in an internship interview.

Enquiry is from Genetic Recombination in section Genetic Recombination of Drug Biotechnology

1 Answer

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The correct choice is (b) Lac, Gal, Azi, Ton

The best explanation: The HFR can be produced by incorporation of the plasmid within the DNA at different positions. So here in two cases, the position is different. Considering the relative time of entry, we can see a pattern which agrees with option 2. It can be in either orientation.

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