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The most common procedures of irreversible enzyme immobilization are covalent coupling, entrapment or micro-encapsulation and ____________

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The most common procedures of irreversible enzyme immobilization are covalent coupling, entrapment or micro-encapsulation and ____________

(a) Hydrophilic linkage

(b) Di-sulphide bonding

(c) Covalent bonding

(d) Cross-linking

I got this question in an internship interview.

Enquiry is from Methods of Immobilization topic in portion Antibiotics & Enzyme Immobilization of Drug Biotechnology

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Right answer is (d) Cross-linking

To explain: The concept of irreversible immobilization means that once the biocatalyst is attached to the support it cannot be detached without destroying either the biological activity of the enzyme or the support. Thus the most common procedures of irreversible enzyme immobilization are covalent coupling, entrapment or micro-encapsulation and crosslinking of the enzyme with polymers.

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