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What is the binding energy?

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What is the binding energy?

(a) Free energy released in the formation of enzyme-substrate interaction

(b) The energy required to form a bond

(c) The energy required to bind substrate

(d) It is the activation energy

I got this question during a job interview.

Origin of the question is Enzymes in division Biomolecules and Catalysis of Life Sciences

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The correct choice is (a) Free energy released in the formation of enzyme-substrate interaction

Easiest explanation: Binding energy is the free energy released when the enzyme-substrate complex is formed. This energy is used to lessen the activation energy. Binding energy can be maximum when the only correct substrate binds to the enzyme.

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