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Which of the following is not used to determine DNA relatedness in the phylogenetic system?

(a) Thermal stability of related DNA

(b) Genome size

(c) GC content

(d) Amino acid sequences

This question was posed to me during an interview.

Query is from Classification System topic in division Prokaryotes and Viruses of Life Sciences

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Right answer is (d) Amino acid sequences

The best I can explain: DNA relatedness can be determined by following five factors: GC content, genome size, the thermal stability of related DNA sequences, DNA relatedness when the condition is optimal for DNA reassociation, and DNA relatedness when the condition is superoptimal for DNA reassociation.

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