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What is the maximum wavelength that Tryptophan and tyrosine absorb?

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What is the maximum wavelength that Tryptophan and tyrosine absorb?

(a) 280nm

(b) 260nm

(c) 257nm

(d) 230nm

I had been asked this question at a job interview.

Origin of the question is Amino Acids and Proteins in section Biomolecules and Catalysis of Life Sciences

1 Answer

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Correct choice is (a) 280nm

Easy explanation: Tryptophan, tyrosine, and phenylalanine are aromatic amino acids and absorbs ultraviolet light (UV). Absorption at 280nm is used for quantification and detection of purified proteins. The absorbance of proteins is dependent on number and position of aromatic amino acid residues.

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