Question

Insertion of recombinant DNA within the gene encoding for β–galactosidase leads to ________

(a) amplification

(b) transformation

(c) insertional inactivation

(d) cloning

The question was posed to me by my school principal while I was bunking the class.

The doubt is from Tools of Recombinant DNA Technology: Cloning Vectors topic in section Biotechnology Principles and Processes of Biology – Class 12

Answer 1

The correct answer is (c) insertional inactivation

For explanation I would say: Insertional inactivation is the process in which the gene is inactivated due to the insertion of other genes within it or within its coding sequence. This leads to loss of function of that specific gene. Thus, insertion of recombinant DNA within the gene encoding for β–galactosidase will lead to insertional inactivation.