Question

The three steps involved in PCR are ________

(a) annealing, extension, and denaturation

(b) extension, denaturation, and annealing

(c) denaturation, annealing, and extension

(d) denaturation, extension, and annealing

The question was asked in examination.

Question is from Biotechnology in section Biotechnology Principles and Processes of Biology – Class 12

Answer 1

Right answer is (c) denaturation, annealing, and extension

For explanation: Denaturation, annealing, and extension are three steps involved in PCR. In denaturation, the DNA is denatured. The primers are added in annealing and in extension, the primers extend giving rise to new DNA strands.