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Which of the following will improve the efficiency of the separation process in liquid chromatography?

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Which of the following will improve the efficiency of the separation process in liquid chromatography?

(a) Increase in sample size, increase in column diameter

(b) Reduction in sample size, increase in column diameter

(c) Increase in sample size, reduction in column diameter

(d) Reduction in sample size, reduction in column diameter

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This intriguing question originated from Liquid Chromatography topic in portion Chromatography of Analytical Instrumentation

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Correct choice is (d) Reduction in sample size, reduction in column diameter

Easy explanation: Reduction in sample size and reduction in column diameter will improve the efficiency of the separation process in liquid chromatography. The effect of uneven flow will also be reduced.

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