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The major problem associated with sample preparation is which of the following?

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The major problem associated with sample preparation is which of the following?

(a) Preparing sample in the right quantity

(b) Choosing sample holders

(c) Matrix effects

(d) Reflection by holders

I had been asked this question during an interview.

This key question is from Total Reflection X-Ray Fluorescence Spectrometer topic in portion X-Ray Spectrometers of Analytical Instrumentation

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Correct option is (c) Matrix effects

The explanation is: The major problem associated with sample preparation is matrix effects. The primary and emitted radiation is absorbed by the element present along with the elements of interest.

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