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Which type of chromatography depends on the principle of size of particles?

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Which type of chromatography depends on the principle of size of particles?

(a) Affinity chromatography

(b) Gel- filtration chromatography

(c) Ion- exchange chromatography

(d) Multimodal chromatography

The question was posed to me during an online exam.

This interesting question is from Chromatography topic in chapter Unit Operations of Bioprocess Engineering

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The correct choice is (b) Gel- filtration chromatography

Easy explanation: Gel filtration chromatography, a type of size exclusion chromatography, can be used to either fractionate molecules and complexes in a sample into fractions with a particular size range, to remove all molecules larger than a particular size from the sample, or a combination of both operations.

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