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According to the small size of the particle, which type of chromatographic separation is applicable?

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in Bioprocess Engineering by (80.7k points)
According to the small size of the particle, which type of chromatographic separation is applicable?

(a) High- performance liquid chromatography (HPLC)

(b) Fast protein liquid chromatography (FPLC)

(c) Gel chromatography

(d) Paper chromatography

This question was addressed to me in unit test.

Asked question is from Chromatography topic in division Unit Operations of Bioprocess Engineering

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Correct choice is (a) High- performance liquid chromatography (HPLC)

To explain I would say: In order to achieve the high resolutions characteristic of HPLC, stationary-phase particles 2-5 μm in diameter are commonly used. Because the particles are so small, HPLC systems are operated under high pressure (5-10 MPa) to achieve flow rates of 1-5 ml min -1. FPLC instruments are not able to develop such high pressures (1-2 MPa) and are therefore operated with column packings of larger size.

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