Question

What is the yield coefficient if the initial substrate concentration is 10 g/l and biomass is 0.1 g/l. The substrate is then consumed and produces 5.3 g/l of biomass?

(a) 0.50 g biomass / g substrate

(b) 0.52 g biomass / g substrate

(c) 0.54 g biomass / g substrate

(d) 0.56 g biomass / g substrate

The question was posed to me by my college director while I was bunking the class.

Origin of the question is Yields in Cell Culture in division Homogenous Reactions of Bioprocess Engineering

Answer 1

Correct answer is (b) 0.52 g biomass / g substrate

Best explanation: Yield = (5.3 – 0.1 g biomass) / (10 g substrate) = 0.52 g biomass/g substrate.