Question

Which of the following is not included in immobilization process?

(a) Absorption

(b) Adsorption

(c) Entrapment

(d) Affinity

I have been asked this question in an interview.

Origin of the question is Large in portion An Overview of Biological Basics & Enzymes of Bioprocess Engineering

Answer 1

Right answer is (a) Absorption

The explanation is: Despite their excellent catalytic capabilities, enzymes and their properties must be improved prior to industrial implementation in many cases. Some aspects of enzymes that must be improved prior to implementation are stability, activity, inhibition by reaction products, and selectivity towards non-natural substrates. This may be accomplished through immobilization of enzymes on a solid material, such as a porous support. Immobilization of enzymes greatly simplifies the recovery process, enhances process control, and reduces operational costs. Many immobilization techniques exist, such as adsorption, covalent binding, affinity, and entrapment.