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Which technique was used to study the secretory pathway in acinar cells of the pancreas for the first time?

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Which technique was used to study the secretory pathway in acinar cells of the pancreas for the first time?

(a) Gel electrophoresis

(b) Chromatography

(c) Autoradiography

(d) ELISA

This question was addressed to me in semester exam.

The doubt is from Cytoplasmic Membrane Systems topic in chapter Cytoplasmic Membrane Systems of Cell Biology

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The correct choice is (c) Autoradiography

The best explanation: The acinar cells of the pancreas secrete digestive enzyme that reach the small intestine and aid in digestion of food. The steps involved in the synthesis of these proteins, migration to cell surface and the subsequent discharge was studied using autoradiography by James Jamieson and George Palade of Rockefeller University.

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