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Which of the following is a Sanger’s reagent?

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Which of the following is a Sanger’s reagent?

(a) 1-fluoro-2, 4-dinitrobenzene

(b) 1-fluoro-2, 3-dinitrobenzene

(c) 1-fluoro-2, 4-trinitrobenzene

(d) 1-fluoro-2, 3-trinitrobenzene

The question was asked in semester exam.

This intriguing question originated from Protein Sequences and Evolution topic in division Amino Acids, Peptides and Proteins of Biochemistry

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The correct choice is (a) 1-fluoro-2, 4-dinitrobenzene

For explanation I would say: To identify the amino-terminal amino acid residue, Sanger developed the reagent 1-fluoro-2, 4-dinitrobenzene (FDNB).

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