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Which of the following is Edman reagent?

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Which of the following is Edman reagent?

(a) Phenylisothiocyanate

(b) CF3 COOH

(c) FDNB

(d) Phenylthiocarbonyl

The question was posed to me in final exam.

My question is from Protein Sequences and Evolution topic in portion Amino Acids, Peptides and Proteins of Biochemistry

1 Answer

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Correct option is (a) Phenylisothiocyanate

For explanation: The Edman reagent, phenylisothiocyanate reacts with the amine group of the N-terminal amino acid.

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