Question

Which of the following cannot be related to multiple sequence alignment?

(a) Many conserved and functionally critical amino acid residues can be identified in a protein multiple alignment

(b) Multiple sequence alignment is also an essential prerequisite to carrying out phylogenetic analysis of sequence families and prediction of protein secondary and tertiary structures

(c) Multiple sequence alignment also has applications in designing degenerate polymerase chain reaction (PCR) primers based on multiple related sequences

(d) This method does not contribute much to degenerate polymerase chain reaction (PCR) primers creation

The question was asked during an internship interview.

I would like to ask this question from Exhaustive Algorithms topic in section Multiple Sequence Alignment of Bioinformatics

Answer 1

Right answer is (d) This method does not contribute much to degenerate polymerase chain reaction (PCR) primers creation

The explanation: In practice, heuristic approaches are most often used. Multiple sequence alignment has applications in designing degenerate (PCR) primers based on multiple related sequences.