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Which of the following enzymes’ combined action leads to the generation of sticky ends in the plasmid vector?

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Which of the following enzymes’ combined action leads to the generation of sticky ends in the plasmid vector?

(a) Alkaline phosphatase and terminal transferase

(b) Exonuclease III and alkaline phosphatase

(c) Bacteriophage lambda exonuclease and terminal transferase

(d) Exonuclease III and terminal transferase

I had been asked this question in an interview.

The doubt is from DNA Cloning: The Basics-1 in portion DNA-Based Information Technologies of Biochemistry

1 Answer

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Best answer
Correct option is (c) Bacteriophage lambda exonuclease and terminal transferase

To elaborate: The combined action of bacteriophage lambda exonuclease and terminal transferase

lead to the generation of sticky ends in plasmid vector.

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