Question

Which of the following is true about Michaelis-Menten kinetics?

(a) Km, the Michaelis constant, is defined as that concentration of substrate at which enzyme is working at maximum velocity

(b) It describes single substrate enzymes

(c) Km, the Michaelis constant is defined as the dissociation constant of the enzyme-substrate complex

(d) It assumes covalent binding occurs between enzyme and substrate

I got this question by my school teacher while I was bunking the class.

This interesting question is from Enzyme Kinetics as an Approach to Understanding Mechanism topic in chapter Enzymes, Carbohydrates and Gycobiology of Biochemistry

Answer 1

The correct answer is (b) It describes single substrate enzymes

To explain: Km is defined as the concentration of substrate at which enzyme is working at half of maximum velocity. It is also a measure of the affinity that the enzyme has for its substrate. Michaelis-Menten kinetics assumes non-covalent binding between enzyme and substrate.