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In the intermediate steps of EM algorithm, the number of each base in each column is determined and then converted to fractions.

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In the intermediate steps of EM algorithm, the number of each base in each column is determined and then converted to fractions.

(a) True

(b) False

I have been asked this question in an interview.

The origin of the question is Statistical Methods for Aiding Alignment in chapter Multiple Sequence Alignment of Bioinformatics

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The correct option is (a) True

Explanation: For example, that there are four Gs in the first column of the 10 sequences, then the frequency of G in the first column of the site, fSG = 4/10 = 0.4. This procedure is repeated for each base and each column.

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