Question

It has been estimated that up to 11% of cDNA clones may be chimeric.

(a) True

(b) False

The question was asked in homework.

My doubt is from Sequence topic in section Functional Genomics & Proteomics of Bioinformatics

Answer 1

The correct answer is:

(a) True

It is indeed estimated that up to 11% of cDNA clones may be chimeric. Chimeric clones occur when DNA fragments from different sources are inadvertently joined together during the cloning process. This can happen when there is incomplete ligation or when fragments from different cDNA molecules are recombined. Chimeric clones can lead to inaccurate data in gene expression studies, so it is important to detect and filter them out during sequencing and analysis.

Thus, the statement is true.