Question

To generate EST data, clones in the cDNA library are randomly selected for sequencing from either end of the inserts.

(a) True

(b) False

I got this question in a job interview.

My question is based upon Sequence topic in chapter Functional Genomics & Proteomics of Bioinformatics

Answer 1

The correct answer is:

(a) True

In the process of generating Expressed Sequence Tags (ESTs), clones from a cDNA library are randomly selected, and sequencing is typically done from either end of the inserts. These sequences provide short fragments that can be aligned to represent the entire transcriptome, which helps in identifying genes and their expression levels. The random selection and sequencing from both ends of the inserts allow for efficient identification and cataloging of genes that are expressed in the organism being studied.

Thus, the statement is true.