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The putative ORF can be translated into a protein sequence, which is then used to search against a protein database.

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The putative ORF can be translated into a protein sequence, which is then used to search against a protein database.

(a) True

(b) False

This question was posed to me in semester exam.

This intriguing question originated from Gene Prediction in Prokaryotes topic in division Gene and Promoter Prediction of Bioinformatics

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Right option is (a) True

Explanation: The putative frame is further manually confirmed by the presence of other signals such as a start codon and Shine–Delgarno sequence. Detection of homologs from this search is probably the strongest indicator of a protein-coding frame.

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