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In a particular enzyme-catalyzed reaction, Vmax = 0.2 mol/sec and Km = 5 mM. Assume the enzyme shows standard Michaelis-Menten kinetics. What is the rate of the reaction when [S] = 10 mM?

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In a particular enzyme-catalyzed reaction, Vmax = 0.2 mol/sec and Km = 5 mM. Assume the enzyme shows standard Michaelis-Menten kinetics. What is the rate of the reaction when [S] = 10 mM?

(a) 0.133

(b) 0.331

(c) 0.233

(d) 0.332

I had been asked this question during an interview.

This key question is from General Reaction Kinetics for Biological Systems topic in section Homogenous Reactions of Bioprocess Engineering

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The correct choice is (a) 0.133

Easiest explanation: v = Vmax[S] / (Km + [S])

= 0.2×10 / (5+10)

= 0.133.

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