Question

Nontransformed cell lines form monolayer whereas transformed cell lines form multilayer.

(a) True

(b) False

I got this question by my school principal while I was bunking the class.

The doubt is from Methods Used for the Cultivation of Animal Cells in chapter Bioprocess Considerations in Using Animal Cell Cultures of Bioprocess Engineering

Answer 1

Right answer is (a) True

Explanation: One particular characteristic is contact inhibition. In two-dimensional culture on a surface nontransformed cells form only a monolayer, as cell division is inhibited when a cell’s surface is in contact with other cells. Transformed cells do not “sense” the presence of other cells and keep on dividing to form multilayer structures.