Question

Resolubilization is generally for the chemical alternations.

(a) True

(b) False

This question was addressed to me in an interview for job.

Origin of the question is Guidelines for Choosing Host-Vector Systems topic in division Utilizing Genetically Engineered Organisms & Medical Applications of Bioprocess Engineering of Bioprocess Engineering

Answer 1

Right option is (b) False

To elaborate: The protein in the inclusion body is misfolded. The misfolded protein has no biological activity and is worthless. If the inclusion bodies are recovered from the culture, the inclusion bodies can be resolubilized and activity restored. When resolubilization is straightforward and recoveries are high, the formation of inclusion bodies can be advantageous, as it simplifies the initial steps of recovery and purification. It is important that during resolubilization the protein be checked by several analytical methods to ensure that no chemical modifications have occurred. Even slight changes in a side group can alter the effectiveness of the product.