Question

The accumulation of toxic metabolic products in beads is a problem of which of the following methods?

(a) Gel beads

(b) Microencapsulation

(c) Tubular ceramic matrix

(d) Roller bottles

I had been asked this question during an interview for a job.

I want to ask this question from Bioreactor Considerations topic in section Bioprocess Considerations in Using Animal Cell Cultures of Bioprocess Engineering

Answer 1

The correct choice is (a) Gel beads

For explanation I would say: High cell densities make high volumetric productivities possible. However, the control of microenvironmental conditions inside bead particles and the accumulation of toxic metabolic products in beads are potential problems in the immobilization of mammalian cells in gel beads (agar, alginate, collagen, polyacrylamide).