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Which technique was used to find the relationship between 15S and 10S globin RNAs?

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Which technique was used to find the relationship between 15S and 10S globin RNAs?

(a) S-loop formation

(b) R-loop formation

(c) Pulsed-field gel electrophoresis

(d) Polymerase chain reaction

I got this question in a national level competition.

My question comes from Gene Expression topic in portion Gene Expression of Cell Biology

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Correct answer is (b) R-loop formation

Explanation: The technique known as R-loop formation was employed by Shirley Tilghman, Philip Leder, and their co-workers at the National Institutes of Health for discerning the relationship between 15S and 10S globin RNAs.

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