Question

The temperature cycles in a polymerase chain reaction are in the order __________________

(a) 95°, 60°, 72°

(b) 60°, 72°, 95°

(c) 72°, 60°, 95°

(d) 95°, 72°, 60°

This question was posed to me by my college director while I was bunking the class.

My question is based upon Techniques topic in division Techniques in Cell Biology of Cell Biology

Answer 1

The correct answer is (a) 95°, 60°, 72°

Easiest explanation: Three different temperatures are reached during the polymerase chain reaction to facilitate the denaturation and hybridization of the DNA strands. First the temperature is elevated to 95°C, then lowered to 60°C and then again increased to 72°C.