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Which is the main ingredient in the sample preparation of mass spectrometry?

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Which is the main ingredient in the sample preparation of mass spectrometry?

(a) papain

(b) pepsin

(c) vinculin

(d) trypsin

I had been asked this question in semester exam.

The doubt is from Techniques topic in section Techniques in Cell Biology of Cell Biology

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The correct answer is (d) trypsin

Easiest explanation: Trypsin is used in a mass spectrometric analysis to digest proteins. After protein digestion, there is a fractionation step using the liquid chromatographic procedures. After that the samples are ionized and made gaseous.

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